Fig. 6: 4HPAA and 3HPP prevent obesity independently of the microbiome.
a, The β diversity of the microbiome in HFD- and ND-fed mice treated with 4HPAA or 3HPP for 7 days (short term). n = 10 mice per group. b, The β diversity of the microbiome in different long-term treatment groups, calculated by principal component analysis (based on weighted UniFrac distances). n = 6 mice per group. c,d, HFD mice that did or did not receive oral 4HPAA or 3HPP treatment were simultaneously treated with an antibiotic cocktail (ABX) for 4 weeks (n = 5 mice per group) and body weight (c) and fat percentage (d) were determined. e, 4HPAA, 3HPP and 4HPP contents in bacterial culture supernatant measured using UPLC–MS; cps, counts per second. f, The ABX-treated pseudosterile mice were administered the indicated bacteria through oral gavage every 3 days for 8 weeks. Violin plots show the body weight gain (n = 4 for C. argentinense group and n = 5 for other groups). Data are shown as mean ± s.e.m. Statistical analysis was performed using the two-tailed Mann–Whitney U test. Experiments were independently repeated twice, with similar results.
