Extended Data Fig. 3: Knockdown of HTR2c in VMHPdyn neurons prevents Olanzapine-induced overweight in female mice.
From: GLP-2 prevents antipsychotics-induced metabolic dysfunction in mice
a, Raw traces and statistical analysis of the effect of CP809101 (50 µM) on VMHPdyn neurons. The statistical analysis illustrates the inter-spike intervals (ISIs) and firing rate change following bath application of CP809101(N = 5 neurons from 4 mice per group). b, Representative traces of the neuronal firing change in the presence (upper) and absence of CP809101 (below) while treated with olanzapine (N = 9 cells from 7 mice per group). c, Quantification (right) of the neuronal firing change (N = 9 cells from 7 mice per group). d, Relative expression level of HTR2c gene in VMH (normalized to GAPDH). e, Change in body weight. f, Change in body composition. g, Changes in blood glucose (left) and the area under the curve (AUC) (right) of mice subjected to ipGTT. h, Changes in blood glucose (left) and AUC (right) of mice subjected to ipITT. i–l, Metabolic cage analysis of food intake (i), physical activity (j), heat production (k) and RER (l). N = 5 mice per group for d–l. Data are plotted as mean ± SEM. P values were determined by Unpaired two-tailed Student’s t-tests in a, d, g(right), h(right), Two-way ANOVA followed by Sidak’s multiple-comparison tests in e, f, g(left), h(left), i–l, followed by Tukey’s multiple-comparison tests in c.
