Extended Data Fig. 5: Homogenous function of VMHPdyn neurons with different projections in male mice.
From: GLP-2 prevents antipsychotics-induced metabolic dysfunction in mice
a, Schematics for laser stimulation of VMHpdyn → VLPO projections. White lines indicate the boundaries of brain structures. (b–d) Average traces of food intake (b), core (c) and BAT (d) temperature after optogenetic activation of VMHpdyn → VLPO projections. (b–d, N = 3 mice per group). Blue lines indicate the time of 5 Hz 15-second laser stimulation. Data are plotted as mean ± SEM. e, Schematics for laser stimulation of VMHpdyn → PAG projections. White lines indicate the boundaries of brain structures. f–h, Average traces of food intake (f), core (g) and BAT (h) temperature after optogenetic activation of VMHpdyn → PAG projections. (f–h, N = 3 mice per group). Blue lines indicate the time of 5 Hz 15-second laser stimulation. Data are plotted as mean ± SEM. i, Schematics for laser stimulation of VMHpdyn → MEA projections. White lines indicate the boundaries of brain structures. Scale bar, 200 mm. j–l, Average traces of food intake (j), core (k) and BAT (l) temperature after optogenetic activation of VMHpdyn → MEA projections. (j–l, N = 3 mice per group). Blue lines indicate the time of 5 Hz 15-second laser stimulation. Data are plotted as mean ± SEM. Shaded area of the graph indicated time points that are statistically different from eYFP group. P values were determined by Two-way ANOVA followed by Sidak’s multiple-comparison test in b–d, f–h, j–l.
