Extended Data Fig. 2: Chemical disruption of heme acquisition represses the thermogenic profile without impairing adipocyte differentiation.
From: Haem biosynthesis regulates BCAA catabolism and thermogenesis in brown adipose tissue
a) mRNA relative expression of adipogenic genes and Alas1 in primary brown adipocytes differentiated in the presence of vehicle, succinylacetone (SA), heme-depleted serum (HDS), or SA + HDS (n = 3 biological replicates per condition). b) Primary brown adipocytes treated as described in a have comparable capacity for adipogenic differentiation. Nile red (red) and Hoechst (blue) used to stain lipid droplets and nuclei, respectively. Scale bar = 100 μm. c) Volcano plot of global protein abundance in primary brown adipocytes treated with SA relative to vehicle (n = 4 biological replicates per condition). d) Bubble plot of biological pathways enriched among significantly changed genes with a log2-fold change less than -1. Data shown as mean ± SD. p values vs. FBS, one-way ANOVA with multiple comparisons and a Tukey’s post-test.
