Extended Data Fig. 8: Neither the locus coeruleus, nor its angiogenic downstream brain regions, are activated by GDF15.
a) Quantification of c-Fos positive cells in the locus coeruleus 90-min following GDF15 treatment (5 nm/kg IP), with representative pictures of the staining for TH and c-Fos. n = 4 per group. Data presented as mean ± s.e.m. with p-values calculated using unpaired two-tail t-test. Scale bar=200 µm. 4 V: 4th ventricle, LC: locus coeruleus, TH: tyrosine hydroxylase. b) Quantification of c-Fos positive cells in the PFC and BLA 90-min following GDF15 treatment (5 nm/kg IP), with representative pictures of the staining. n = 4 per group. Data presented as mean ± s.e.m. with p-values calculated using unpaired two-tail t-test. Scale bar=200 µm. BLA: basolateral amygdala, cc: corpus callosum, mPFC: medial prefrontal cortex. c) Serum GDF15 in WT (control n = 9, restraint n = 9) and Gfral -/- mice (control n = 8, restraint n = 8). Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA with Tukey’s post-hot correction. d) Serum corticosterone in WT (control n = 4, CRH n = 6) and Gfral -/- mice (control n = 4, CRH n = 6). Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA at each timepoint. e) Serum adrenocorticotropic hormone (ACTH) in WT (control n = 4, CRH n = 6) and Gfral -/- mice (control n = 4, CRH n = 6). Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA at each timepoint. f) Serum corticosterone 6-hr post treatment with dexamethasone (Dexa, 100 µg/kg IP). n = 4 per group. Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA.
