Extended Data Fig. 5: Macrophages are the most likely source of GDF15 within adipose tissue.
a) Fgf21 expression in mouse adipocyte and SVF from gWAT post-saline (n = 4 per group) or epinephrine (n = 5 per group). Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA with post-hoc test and Tukey’s correction. b) Leptin expression in mouse adipocyte and SVF from gWAT post-saline (n = 3 per group) or epinephrine (n = 4 per group). Data presented as mean ± s.e.m. with p-values calculated using 2-way ANOVA. c) t-SNE plot of Lin- stromal vascular cells from gWAT of control mice and mice treated with CL for 3 days. Clustering identified 6 major cell types/states. Clusters are highlighted in different colors. The data set was queried for cells expressing Gdf15. Heatmap shows expression of Gdf15 and other cell-identifying factors in the various identified cell populations. d) F480/Adgre1 expression in mouse F480+ and F480- fractions of SVF from gWAT 1-hr post-epinephrine treatment (0.5 mg/kg). Data presented as mean ± s.e.m. with n = 3 per group. p-values calculated using 2-way ANOVA. e) Arg1 and Nos2 expression in bone marrow-derived macrophages (BMDMs) polarized to either M1-like or M2-like. n = 6 per group. Individual data points represent duplicates from 3 independent experiments. Data presented as mean ± s.e.m. with p-values calculated using 1-way ANOVA with post-hoc test and Tukey’s correction. f) GDF15 levels in media from BMDMs polarized to either M1-like or M2-like for 24-hrs. n = 3 per group. Individual data points represent triplicates from 3 independent experiments. Data presented as mean ± s.e.m. with p-values calculated using 1-way ANOVA with post-hoc test and Tukey’s correction. g) GDF15 levels in media from M2-like BMDMs treated with epinephrine (1 μM) or tunicamycin (5 ng/mL) for 24-hrs. n = 3 per group. Individual data points represent triplicates from 3 independent experiments. Data presented as mean ± s.e.m. with p-values calculated using 1-way ANOVA with post-hoc test and Tukey’s correction. h) Volcano plot showing fatty acid transporters identified between M1- and M2-like macrophage populations from scRNA-seq data.
