Fig. 5: Hepatic gluconeogenesis contributes to cachexia in Yki flies.
From: Hepatic gluconeogenesis and PDK3 upregulation drive cancer cachexia in flies and mice
a,b, The climbing ability at day 6 (a) (n = 4) and survival curve (b) (n = 3) of control flies, Yki flies, and Yki flies with fat-body hop or Pdk depletion. c, Whole-body trehalose levels of control flies, Yki flies, and Yki flies with fat-body Tps1 depletion at day 8 (n = 4). d,e, The climbing ability at day 7 (d) (n = 4) and survival curve (e) (n = 3) of control flies, Yki flies, and Yki flies with fat-body Tps1 depletion. f, An illustration of the metabolic pathway of the TCA cycle and gluconeogenic amino acids. g, The experimental design of metabolomics analysis. h–j, Relative levels of whole-body alanine (h), phenylalanine (i), and leucine and isoleucine (j) (n = 4); data were retrieved from metabolomics analysis. k–p, Relative levels of abdomen citrate (k), alanine (l), phenylalanine (m), leucine and isoleucine (n), methionine (o), and α-ketoglutarate (p) (n = 4); data were retrieved from metabolomics analysis. q,r, Violin plots of expression levels of ScsβA at day 5 (q) and day 8 (r) in the fat-body cell cluster; data were retrieved from snRNA-seq. Box plots denote the median (bottom centre line), mean (top centre line), and interquartile range. The whiskers of each box plot extend to the lowest and highest expression levels. s, qRT–PCR analysis of ScsβA in the fat body of control flies, Yki flies, and Yki flies with fat body hop depletion at day 6 (n = 3). t, qRT–PCR analysis of ScsβA in the fat body of Yki flies and Yki flies with fat body Stat92e depletion at day 6 (n = 3). u, Relative levels of circulating (haemolymph) phenylalanine (n = 4); data were retrieved from metabolomics analysis. v, Relative levels of thoraces (muscle) phenylalanine (n = 4); data were retrieved from metabolomics analysis. Statistical significance was assessed by unpaired two-sided Student’s t-test (t) and ordinary one-way ANOVA (a,c,d,h–p,s,u,v). Error bars indicate s.d., with the mean as the centre. See also Extended Data Fig. 5.
