Extended Data Fig. 1: Related to Figure 1.
From: Hepatic gluconeogenesis and PDK3 upregulation drive cancer cachexia in flies and mice
. (a) Total number of nuclei detected in each cell cluster across all samples retrieved from snRNA-seq. (b) Dot plot showing marker genes of each cell cluster. Color scale indicates Z-score, and size indicates expression percentage. Dot plot showing the change in cell proportion of each cell cluster between Yki and control at Day 5 (c) and Day 8 (d). Cell proportions were normalized to each condition and the Log2 ratio Yki vs control cell counts were calculated. The size of each dot represents the number of cells in Yki flies, and the plot is ordered by descending Log2 ratio. Bar plots showing the number of DEGs in each cluster (using a Wilcoxon Rank Sum test with p-value < 0.05 and abs (Log2FC) > 0.38) at Day 5 (e) and Day 8 (f). The top and bottom five clusters are labelled. Flyphone prediction of cell-to-cell communication at Day 5 (g) and Day 8 (h). The interaction scores of tumor cells (05_intestinal_stem_cell and 06_enterocyte) and peripheral tissues was calculated. The difference in interaction score between Yki and control was taken and ligand-receptor pairs which did not express a DEG in either the tumor or host tissue between Yki and control were filtered out. The width of each connection reflects the number of ligand-receptor pairs between tumor and host.
