Fig. 4: Neurospheres retain their metabolic signatures as orthotopically implanted xenografts, and these signatures correlate with drug response. | Nature Metabolism

Fig. 4: Neurospheres retain their metabolic signatures as orthotopically implanted xenografts, and these signatures correlate with drug response.

From: Cell-intrinsic metabolic phenotypes identified in patients with glioblastoma, using mass spectrometry imaging of 13C-labelled glucose metabolism

Fig. 4

a, Representative MSI sections from rat brains implanted with AT8, S2 and AT5 (n = 3 independent tumours per model). Relative signal intensities for [U-13C]lactate (top) and [13C2]glutamate (bottom); H&E-staining of the corresponding sections. b, The relative signal intensities of [U-13C]lactate (****P < 0.0001) and [13C2]glutamate (AT8 vs AT5, P = 0.0012; S2 vs AT5, P = 0.0406; AT8 vs AT5, P = 0.0012) in neurospheres (AT8, n = 4; S2, n = 5; AT5, n = 4) and the respective xenografts (AT8, n = 3; S2, n = 3; AT5, n = 3) expressed as mean values; error bars, s.d. ([13C2]glutamate AT8 vs AT5, P = 0.0015; S2 vs AT5, P = 0.0049). *P < 0.05; **P < 0.005. c, Relative abundance (mean values; error bars, s.d.) of labelled malate and fumarate in sections of A11 and S2 xenografts. d, Top: labelled malate and fumarate signal intensities in MS images of the brains of rats implanted with A11 (n = 12) and S2 (n = 12) xenografts. Bottom: H&E-staining of the corresponding sections. Asterisks refer to P values obtained from one-way ANOVA followed by Tukey’s multiple comparisons test or unpaired t-test (****P < 0.0001).

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