Extended Data Fig. 1: Analysis of lactate and glutamate labelling in GB1 and GB2.
a) Coronal MR images from the two infused GB patients showing the locations of the sampled regions (blue stars). There were no significant differences in the mean signal intensities (± SD) of [13C2]glutamate and [U-13C]lactate in the core and periphery of GB1 (core=9; periphery =13) and GB2 (core=8; periphery =4) (unpaired T-test). b) Labelling of lactate and TCA cycle intermediates from [U-13C]glucose. c) Simple linear regression of the [13C2]glutamate signal intensities against the intensities of labelled TCA cycle metabolites. Each dot represents the mean intensity of the metabolite per tumour section (13C2 malate: R2 = .55, F(1, 51) = 62.56, p < 0.0001; 13C2 fumarate: R2 = .45, F(1, 51) = 41.51, p < 0.0001; 13C2 succinate: R2 = .39, F(1, 51) = 32.05, p < 0.0001) d) MALDI-MSI images of C1P(44:3) intensity across the three different patient tumours. The first section from GB1 was located at the beginning of the slide and was imaged first while the last metastasis section was located at the end of the slide and was imaged last. Tumour section locations on the slides were randomised.
