Extended Data Fig. 2: Assessment of tumour bioenergetics and analysis of plasma metabolites.
a) 31P NMR spectrum of a patient’s tumour extract. The sample was collected using pituitary ronguer followed by rapid intra-operative snap freezing. b) ATP/ADP ratios determined from 31P NMR measurements on extracts from three patients tumours. The samples were frozen immediately or with 5 second delay. c) Relative ATP (GB1 vs met p = 0.0001; GB2 vs met p = 0.0329) and PCr (GB1 vs met p = 0.0253; GB2 vs met p = 0.047) signal intensities in each tumour section. Asterisks refer to P values obtained from a one way ANOVA test followed by Tukey’s multiple comparisons test. d) Arterial blood concentrations of unlabelled (top) and labelled (bottom) metabolites, measured using LC-MS, in the three patients during [U-13C]glucose infusion. e) K-means segmentation, based on lactate and glutamate 13C-labelling, of the adenocarcinoma metastasis into tumour (red), gliotic brain (blue), and normal brain (yellow). Corresponding H&E sections are shown (acquired post-DESI-MSI). Adenocarcinoma, gliotic brain and normal cortex are outlined. The H&E sections were annotated by a neuro histopathologist.
