Extended Data Fig. 4: Correlation between the mass spectrometry images and spatial transcriptomics.
a) Spatial transcriptomic images were segmented using k-means into three metabolic groups, with blue representing highly glycolytic cells (expressing Hallmark glycolytic gene sets), yellow representing oxidative cells (expressing Hallmark oxidative gene sets) and red representing cells low for both glycolysis and oxidative phosphorylation transcripts. b) Percent concordance between MSI and RNA derived metabolic states for all tumour pixels. c) ADP signal intensities (mean ± SD) were significantly higher in the oxidative regions of GB defined by the MSI data (Normal n = 3; Region 1 n = 12; Region 2 n = 4; Region 3 n = 7). Asterisks refer to P values obtained. from one way ANOVA test followed by Tukey’s multiple comparisons test (*P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.00005).
