Extended Data Fig. 2: Validation of Per-Cre-mediated deletion of Gipr in Chow-fed male and female C57BL6/J mice.
Expression of peripherin corrected by the housekeeping gene peptidylprolyl isomerase A (Ppia) in hippocampus (n = 6), hindbrain (n = 6), hypothalamus (n = 5), sciatic nerve (n = 6), trigeminal ganglion (n = 6), dorsal root ganglion (n = 5) and iWAT (n = 4) of 15-week old male chow-fed Per-Cre+Giprwt/wt mice, and in pancreas (n = 7) of 45-week old male chow-fed Per-Cre+Giprwt/wt mice (a). Expression of peripherin corrected by the housekeeping gene Ppia in the cerebellum (n = 8), kidney (n = 8), cerebral cortex (n = 8), midbrain (n = 8), testis (n = 8), pituitary (n = 7), adrenal gland (n = 8), stomach (n = 8), duodenum (n = 8), jejunum (n = 8), ileum (n = 8), colon (n = 8) and trigeminal ganglion (n = 7) of 12-week old male chow-fed WT mice (b). Expression of Gipr in dorsal root ganglion (n = 9 WT, n = 6 KO) (c), hypothalamus (n = 12 WT, n = 10 KO) (d), hindbrain (n = 12 each group) (e), sciatic nerve (n = 12 WT, n = 10 KO) (f), eWAT (n = 12 each group) (g) and pancreas (n = 12 each group) (h) of 45-week old male mice Chow-fed Per-Cre+Giprwt/wt (WT) and Per-Cre+Giprflx/flx (KO) mice. Expression of Gipr in the cerebellum (n = 8 each group) (i), cerebral cortex (n = 8 each group) (j), pituitary (n = 7 WT, n = 8 KO) (k), kidney (n = 8 each group) (l), duodenum (n = 7 WT, n = 6 KO) (m), jejunum (n = 8 each group) (n), ileum (n = 7 WT, n = 8 KO) (o) and colon (n = 8 each group) (p) of 12-week old male mice Chow-fed WT and KO mice. RNAscope validation of Gipr deletion in trigeminal ganglion of 44-week old male chow-fed WT and KO mice (q) and of the DRG of 51-week old male chow-fed WT and KO mice (r). Data in panels q and r are representative examples of n = 3 mice each group. Data in panel c-f and h-p were analyzed using two-sided, two-tailed Student’s ttest, data in panel g were analyzed two-sided by Mann-Whitney test. Data represent mean ± SEM; asterisks indicate * p < 0.05; ** p < 0.01 and *** p < 0.001. Individual p-values are shown in the Data Source file, unless p < 0.0001. Scale bars in panel q and r are 5 μm.
