Extended Data Fig. 3: Metabolic phenotype of HFD-fed female Per-Gipr KO mice.
Body weight development of female C57BL6/J Per-Cre+Giprwt/wt (WT) and Per-Cre+Giprflx/flx (KO) mice fed with a HFD (n = 8 each group) (a). Fat and lean tissue mass of 35-week old female WT and KO mice (n = 8 each group) (b,c). Cumulative food intake of 52-week old female WT (n = 6) and KO mice (n = 7) (d). Energy expenditure (e), locomotor activity (f) and respiratory exchange ratio (RER) (g) of 52-week old female WT (n = 6) and KO mice (n = 7). Glucose tolerance (h) and corresponding area under curve (AUC) (i) after i.p. dosing with 2 g/kg glucose in 48-week old female WT and KO mice (n = 7 each group). Glucose-induced insulin secretion (j) and corresponding AUC (k) after oral glucose bolus administration of 4 g/kg glucose in 54-week old female WT and KO mice (n = 6 each group). Insulin tolerance (l) after i.p. dosing with 0.75U/kg insulin (Humalog) in 50-week old female WT (n = 6) and KO mice (n = 8). Fasting levels of blood glucose (n = 8 each group) (m) and insulin (n = 6 WT, n = 7 KO) (n) in 54-week old female WT and KO mice, as well as fasting plasma levels of triglycerides (n = 6 WT, n = 7 KO) (o) and cholesterol (n = 6 WT, n = 7 KO) (p) in 55-week old female WT and KO mice. Data in panel a,c,f and g were analyzed by repeated measures 2-way ANOVA with Bonferroni’s post-hoc test for comparison of individual time points. Data in panel f, and g, were analyzed using Mann-Whitney test. Data in panel a,d,e,h,j and l were analyzed using 2-way ANOVA and with Bonferroni post-hoc comparison of individual time points. Data in panel e was analyzed using ANCOVA with body weight as covariate. Data in panel b,c,i,k,m-p were analyzed using Student’s two-tailed, two-sided ttest. Data represent mean ± SEM; asterisks indicate * p < 0.05; ** p < 0.01 and *** p < 0.001. Individual p-values are shown in the Data Source file, unless p < 0.0001.
