Fig. 5: snRNA-seq of DVC and hypothalamus of mice treated with incretin mimetics. | Nature Metabolism

Fig. 5: snRNA-seq of DVC and hypothalamus of mice treated with incretin mimetics.

From: GIPR agonism and antagonism decrease body weight and food intake via different mechanisms in male mice

Fig. 5

a,b, A schematic of the experimental design (a) and the body weight (b) of 36-week-old male C57BL/6J WT mice fed either a standard chow diet (cntrl.) or a HFD and treated s.c. with a single dose of either vehicle (vhcl), acyl-GIP (150 nmol kg−1), acyl-GLP-1 (50 nmol kg−1), an acylated peptide GIPR antagonist (1,500 nmol kg−1) or the GIPR:GLP-1R co-agonist MAR709 (50 nmol kg−1) (n = 6 each group). c–f, UMAP representations of gene expression coloured by C12-level cell type in the DVC (c) and C25-level cell type in hypothalamus (d), as well as by experimental group in the DVC (e) and hypothalamus (f). g, A bar graph showing mean expression of Glp-1r and Gipr in the DVC and hypothalamus. h, The number of nuclei isolated from each brain region. The colours correspond to log-normalized expression values scaled to the maximum of each gene. Data in b represent mean ± s.e.m. OPC, oligodendrocyte progenitor cell.

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