Fig. 7: Differential effects of GIPR (ant)agonism on DVC neuronal cell types.
a–d, Bar plots and UMAP representations of gene expression in DVC neurons of male DIO mice treated with a GIPR antagonist (ant.) (a), acyl-GLP-1 (b), acyl-GIP (c) or MAR709 (d) (n = 6 mice per group, from which n = 3 mice were pooled to receive n = 2 independent biological replicates per group). The bar plots and UMAPs are coloured by Augur score, representing cell type-specific changes in gene expression of the treatment group relative to DIO vehicle controls. e–g, Volcano plots (log2 fold change (FC) versus adjusted P values from a two-sided Wilcoxon rank-sum test, corrected for multiple comparison) of differentially expressed genes (DEGs) following treatment with either the GIPR antagonist or acyl-GLP-1 in the top GIPR antagonist affected neuronal clusters Glut10 (e), GABA4 (f) and GABA3 (g). Only the top 15 DEGs are highlighted. Venn diagrams show the overlap of significant DEGs (adjusted P < 0.05) from GIPR antagonist and acyl-GLP-1 groups. P values of DEGs were obtained by Wilcoxon rank-sum tests and were adjusted for multiple comparisons using the Benjamini–Hochberg method.
