Fig. 6: Cysteine deficiency reverses high-fat-diet-induced obesity in mice.
From: Cysteine depletion triggers adipose tissue thermogenesis and weight loss
Cth−/− mice that had been fed HFD for 12 weeks were switched to HFD-CTRL or HFD-CysF. a, Percentage body weight change after switching to HFD-CysF diet (n = 6 Cth−/− HFD-CTRL, n = 5 Cth−/− HFD-CysF and n = 5 Cth+/+ HFD-CysF). b, Fasting blood glucose measured 1 week post diet switch (Cth−/− HFD-CTRL n = 19, Cth−/− HFD-CysF, n = 20). c, The glucose tolerance test (GTT) in Cth−/− after diet switch from HFD-CTRL to HFD-CysF (Cth−/− HFD-CTRL n = 19, Cth−/− HFD-CysF, n = 20). The glucose administration is based on total body weight. d, EE of Cth−/− mice fed with HFD-CTRL or HFD-CysF, average values of nights 4 and 5 of diet switch (n = 6 Cth−/− HFD-CTRL, n = 5 Cth−/− HFD-CysF). e, RER measured in metabolic chambers on days 4 and 5 of diet switch (n = 6 Cth−/− HFD-CTRL, n = 5 Cth−/− HFD-CysF). f, Representative histological sections of SFAT and VFAT stained for UCP1, 6 days after diet switch. g, qPCR analysis of inflammatory genes in CD11b+ F4/80+ VFAT macrophages of Cth−/− mice after diet switch to HFD-CTRL or HFD-CysF (n = 4 per group). Data are expressed as mean ± s.e.m. Statistical differences were calculated by two-way ANOVA with Sidak’s correction for multiple comparisons or by unpaired two-tailed t-tests.
