Extended Data Fig. 6: A rapid and robust activation of signaling through MKK3/4/6, p38, and MK2 occurs specifically in response to resistance exercise in mice.
a, Schematic of how C57BL6 mice were subjected to endurance exercise with treadmill running (TR), resistance exercise with weight pulling (WP), or their respective mock-trained (control) conditions. b, FDL muscles from the mice were collected immediately after the last training bout and subjected to western blot analysis for the phospho (P) and total (T) levels of the indicated proteins. Long isoform of MK2 (L), short isoform of MK2 (S). c, For each sample, the phospho to total protein ratio (P/T) for each signaling event was determined and expressed relative to the mean value observed in the treadmill control group. Values in the graphs are presented as the group mean ± SEM, the number of samples per group is indicated at the bottom of the bars in the graphs. The data were analyzed with two-way ANOVA or a Student’s t-test. ■ Significant difference between the TR control and TR trained group when the planned comparison was analyzed with a Student’s t-test, P < 0.05. The P-value for each statistically significant pairwise comparison is annotated in the graphs with a * being used when P < 0.0001.
