Extended Data Fig. 7: Quantitative results from the western blots in Fig. 7.

FDL muscles from the experimental conditions described in Fig. 7a were subjected to western blot analysis as shown in Fig. 7e. a-k, For each sample, the phospho to total protein ratio (P/T) for each signaling event was determined and then expressed relative to the mean value observed in the treadmill (TR) control group. Values in the graphs are presented as the group mean ± SEM, the number of samples per group is indicated at the bottom of the bars in the graphs. The data were analyzed with two-way ANOVA. The P-value for each statistically significant pairwise comparison is annotated in the graphs with a * being used when P < 0.0001.