Extended Data Fig. 3: MSVGLUT2 neurons are not activated upon exposure to non-edible objects.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a) MSVGLUT2 calcium dynamics and area under the curve (AUC) aligned to the onset of the first sniff when investigating a pellet of food (NCD), wooden stick (familiar object), a small plastic lid (small novel object, first exposure) or a metal teaball (big novel object, first exposure) [time curve: paired two-way ANOVA, Sidak post-hoc; n = 11 mice for sniffing food pellet and metal teaball, n = 12 mice for sniffing wooden stick and small plastic lid] [AUC: mixed effects multiple comparisons analysis; n = 11 mice for sniffing food pellet and metal teaball, n = 12 mice for sniffing wooden stick and small plastic lid; p = 0.0006, p = 0.0004, p < 0.0001]. b) MSVGLUT2 calcium dynamics and area under the curve (AUC) aligned to the drop of the non-edible objects (wooden stick, brown; metal teaball, grey; falcon lid, blue) or normal chow diet pellets (food, orange) into the experimental setup [time curve: paired two-way ANOVA, Sidak post-hoc; n = 13] [AUC: one-way ANOVA, Tukey post-hoc; n = 13 mice; p = 0.002, p = 0.037, p = 0.0002]. c) MSVGLUT2 calcium dynamics and AUC during walking, grooming and rearing behaviour during control exposure aligned to the onset of the behaviour [time curve: n = 6 mice for rearing, n = 13 mice for walking and grooming behaviour] [AUC: one-way ANOVA, Tukey post-hoc; n = 6-13 mice]. Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and, ****p ≤ 0.0001 Mouse images in c reproduced from SciDraw under a Creative Commons license CC BY 4.0. Panels a and b created using BioRender.com.
