Extended Data Fig. 7: MSVGLUT2 neuronal activity in response to non-food related odours is not altered by metabolic status.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a-b) Calcium dynamics and area under the curve (AUC) of MSVGLUT2 neurons aligned to the onset of the first sniff of random fed mice in response to a) limonene compared to control exposure (empty bottle) [AUC: paired two-tailed Student’s t-test; n = 7 mice], or b) geraniol compared to control exposure (empty bottle) [time curve: paired two-way ANOVA, Sidak post-hoc; n = 7 mice] [AUC: paired two-tailed Student’s t-test; n = 7 mice]. c-d) Calcium dynamics and area under the curve (AUC) of MSVGLUT2 neurons aligned to the onset of the odour exposure of random fed mice to c) limonene compared to control exposure (empty bottle) [time curve: paired two-way ANOVA, Sidak post-hoc; n = 7 mice] [AUC: paired two-tailed Student’s t-test; n = 7], or d) geraniol compared to control exposure (empty bottle) [time curve: paired two-way ANOVA, Sidak post-hoc; n = 7 mice] [AUC: paired two-tailed Student’s t-test; n = 7 mice]. e-f) Comparison of MSVGLUT2 calcium dynamics and area under the curve (AUC) aligned to e) the onset of a sniff and f) the onset of stimulus in response to control odour (empty bottle, black), limonene (purple) and geraniol (turquoise) exposure in mice fasted overnight for 16 hours or in random fed mice [time curve: two-way ANOVA, Sidak post-hoc; n = 7 fed mice, n = 9 fasted mice] [AUC: paired two-tailed Student’s t-test; n = 7 fed mice, n = 9 fasted mice]. Lines on top of graphs indicate time points with significant differences in calcium signal between conditions. Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and, ****p ≤ 0.0001. Mouse images in e and f adapted from SciDraw under a Creative Commons license CC BY 4.0. Panels a and b created using BioRender.com.
