Extended Data Fig. 9: Optogenetic stimulation of OB→MS projections did not alter locomotion or anxiety-like behaviour.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a) Schematic of the pre-stimulation protocol employed for OB→MS projections optogenetic stimulation in Tbx21ChR-tdTomato (ChR2). b-d) Cumulative food intake of Tbx21ChR2-tdTomato mice starting at the onset of dark cycle following a 10-minute pre-stimulation before dark cycle after b) three hours, c) six hours d) twelve hours [paired two-tailed Student’s t-test; n = 10 mice]. e) Cumulative food intake and AUC of Tbx21-Cre negative control animals starting at dark cycle onset following a 10-minute pre-stimulation before the dark cycle [time curve: two-way ANOVA, Sidak post-hoc; n = 5 mice] [AUC: two-tailed Student’s t-test; n = 5 mice]. f-h) Cumulative food intake of Tbx21-Cre negative control animals f) three hours, g) after six hours and h) after twelve hours after dark cycle onset following a 10-minute stimulation before dark cycle [two-tailed Student’s t-test; n = 5 mice]. i-p) Open field test 60 minutes after a 10-minute stimulation in Tbx21ChR-tdTomato (ChR2) and Tbx21-Cre negative (control) mice depicted as i) minutes spent per area [two-way ANOVA, Tukey post-hoc; n = 6 Control mice, n = 9 ChR2 mice], j) number of visits per area [two-way ANOVA, Tukey post-hoc; n = 6 Control mice, n = 9 ChR2 mice], k) total distance travelled [two-tailed Student’s t-test; n = 6 Control mice, n = 9 ChR2 mice], l) latency to enter the opposing zone [two-tailed Student’s t-test; n = 6 Control mice, n = 9 ChR2 mice], m) velocity of travel [two-tailed Student’s t-test; n = 6-9], n) distance travelled per area [two-way ANOVA, Tukey post-hoc; n = 6 Control mice, n = 9 ChR2 mice], o) number and p) minutes of rearing [two-tailed Student’s t-test; n = 6 Control mice, n = 9 ChR2 mice]. q-u) Blood collection 60 minutes after a 10-minute stimulation in Tbx21ChR-tdTomato (ChR2) and Tbx21-Cre negative (control) mice. r) Food intake, s) blood glucose levels, t) serum leptin and u) serum cholecystokinin (CCK) levels 60 minutes after a 10-minute stimulation [two-tailed Student’s t-test; n = 5 Control mice, n = 6 ChR2 mice]. Data are represented as mean ± SEM. Eppendorf image in q and mouse images in a and e adapted from SciDraw under a Creative Commons license CC BY 4.0.
