Extended Data Table. 10: Optogenetic stimulation of OB→MS projections did not induce a change in feeding behaviour in diet-obese mice.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a) Bodyweight of CD-fed (lean) and HFD-fed (obese) mice when performing the food finding test [two-tailed Student’s t-test; n = 20 mice]. b) Representative images of the dorsal olfactory bulb (dOB) and quantification comparison of food odour-induced (HFD) pS6 immunoreactivity (pS6, green; Neurotrace, blue) of the dOB, medial olfactory bulb (mOB) and ventral olfactory bulb (vOB) [Student’s t-test; n = 5 mice for control, n = 8 mice for food odours]. Scale bar: 100 µm. c) Cumulative food intake of obese Tbx21ChR2-tdTomato mice following a 10-minute stimulation prior to the dark cycle after d) three hours, e) six hours and f) twelve hours [two-tailed Student’s t-test; n = 7 mice per group]. g) Bodyweight of Tbx21ChR2-tdTomato mice before and after two weeks on HFD. h) Cumulative food intake and AUC of Tbx21-Cre negative control obese animals following a 10-minute pre-stimulation before the dark cycle [time curve: two-way ANOVA, Sidak post-hoc; n = 4 mice per group] [AUC: two-tailed Student’s t-test; n = 4 mice per group]. Cumulative food intake of Tbx21-Cre negative control animals after i) three hours, j) after six hours and k) after twelve hours following a 10-minute stimulation before dark cycle [two-tailed Student’s t-test, n = 4 mice per group]. l-r) Open field test 60 minutes after a 10-minute stimulation of obese Tbx21ChR2-tdTomato (ChR2) and Tbx21-Cre negative (control) mice during the light cycle depicted as l) minutes spent per area [two-way ANOVA, Tukey post-hoc; n = 3 control mice, n = 4 ChR2 mice], m) latency to enter the opposing area [two-tailed Student’s t-test; n = 3 control mice, n = 4 ChR2 mice], n) number and o) minutes of rearing [two-tailed Student’s t-test; n = 3 control mice, n = 4 ChR2 mice], p) velocity [two-tailed Student’s t-test; n = 3 control mice, n = 4 ChR2 mice], q) number of visits and r) distance travelled per area [two-way ANOVA, Tukey post-hoc; n = 3 control mice, n = 4 ChR2 mice]. s) Corticosterone level in blood serum 60 minutes after a 10-minute stimulation of obese Tbx21ChR-tdTomato (ChR2) and Tbx21-Cre negative (control) mice during the light cycle [two-tailed Student’s t-test; n = 5 control mice, n = 9 ChR2 mice]. Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and, ****p ≤ 0.0001. dOB, dorsal olfactory bulb; mOB, medial olfactory bulb; vOB, ventral olfactory bulb; GL, glomerular layer; MTCL, mitral and tufted cell layer. Eppendorf image in s and mouse images in b, c and h adapted from SciDraw under a Creative Commons license CC BY 4.0.
