Fig. 4: Olfactory perception of food increases the activity of MSVGLUT2 neurons independent of palatability.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a, Schematic of the olfactory stimulation chamber allowing for the controlled influx and efflux of odorized air through an olfactometer-based system. A PID measured the amount of volatiles introduced into the system. b, Volatile concentration in the olfactory stimulation chamber during 1 min of food odour exposure of either NCD (orange) or HFD (red) followed by 5 min of exposure to the control odour (empty bottle; black; time curve: n = 3 trials; AUC: repeated-measures one-way ANOVA; n = 3 trials; P = 0.0002, P = 0.001). a.u., arbitrary units. c, Time spent sniffing in the olfactory stimulation chamber during food odour exposure (NCD; orange) or control exposure (black) normalized to the duration of stimulation (repeated-measures one-way ANOVA; n = 4 mice, three repetitions of food odours exposure; P = 0.0387). d–g, Calcium dynamics and AUC of MSVGLUT2 neurons aligned to the onset of the first sniff in response to NCD odours or control exposure (empty bottle; time curve: paired two-way ANOVA, Sidak post hoc; n = 13 mice; AUC: paired two-tailed Student’s t-test; n = 13 mice; P = 0.0006; d), HFD exposure when mice were naive (HFDNaive) or not naive to HFD (HFDNon-naive) (time curve: paired two-way ANOVA, Sidak post hoc; n = 13 mice for HFDNaive, n = 15 mice for HFDNon-naive; AUC: paired two-tailed Student’s t-test; n = 13 mice for HFDNaive, n = 15 mice for HFDNon-naive; e), NCD or control re-exposure in HFDNon-naive mice (time curve: paired two-way ANOVA, Sidak post hoc; n = 12 mice; AUC: paired two-tailed Student’s t-test; n = 12 mice; P = 0.0014; f) and isoamyl acetate or control exposure (time curve: paired two-way ANOVA, Sidak post hoc; n = 13 mice; AUC: paired two-tailed Student’s t-test; n = 13 mice; g). Lines on top of graphs indicate time points with significant differences in calcium signal between conditions. Data are represented as the mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. Panels d–g created using BioRender.com.
