Fig. 6: The MS receives direct projections from the OB.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a, Schematic of anterograde AAV-based mapping of MTC projections in Tbx21-Cre mice. b–d, Representative images of AAV9-FLEX-Arch-GFP-expressing cells (GFP, green; DAPI, blue) in the OB (scale bar, 250 µm; b), the LOT (scale bar, 100 µm; c) and the MS (scale bars, 100 µm; d). e, Schematic of anterograde mapping of MTC projections in Tbx21synaptophysin-tdTomato mice. f–h, Representative images of synaptophysin-tdTomato-expressing cells (tdTomato, red; DAPI, blue) in the OB (scale bar, 250 µm; f), the LOT (scale bar, 100 µm; g) and the MS (scale bars, 100 µm; h). i, Schematic of retrograde rabies-based monosynaptic tracing of projections onto glutamatergic neurons in the MS in VGLUT2-Cre mice. j, Representative images of the injection site, that is, the MS (GFP, green; mCherry, red; DAPI, blue; scale bar, 100 µm). k–p, Representative images of cells projecting onto MSVGLUT2 neurons (mCherry, red; DAPI, blue) in the anterior (scale bar, 50 µm; k) and posterior (scale bar, 100 µm; l) OB, the preoptic area (scale bar, 100 µm; m), the hippocampus (scale bar, 100 µm; n), the lateral hypothalamus (scale bar, 100 µm; o) and the raphe nucleus (scale bar, 100 µm; p). Mouse images in a, e and i adapted from SciDraw under a Creative Commons license CC BY 4.0.
