Fig. 8: Diet-induced obesity blunts OB→MS anorexigenic effects.
From: A food-sensitive olfactory circuit drives anticipatory satiety
a, Food finding test in fasted control diet-fed (lean, black) and HFD-fed (obese, black) C57BL/6N mice (unpaired two-tailed Student’s t-test; n = 10 mice; P = 0.0037). b, Representative images of the MS and quantification comparison of food odour-induced (HFD) phosphorylated S6 (pS6)-IR in the dLS, iLS and MS in fasted C57BL/6N mice after 90 min (two-way ANOVA, Sidak post hoc; n = 6 mice for control, n = 8 mice for food odours; pS6, red; NeuroTrace, blue). Scale bars, 100 µm. c, Cumulative food intake and AUC following 10-min pre-stimulation before the dark cycle in optogenetically stimulated obese Tbx21ChR2-tdTomato (stimulation, blue line) and control (no stimulation, grey/black line) mice (time curve: paired two-way ANOVA, Sidak post hoc; n = 7 mice; AUC: unpaired two-tailed Student’s t-test, n = 7 mice). d, Open field test 60 min following a 10-min stimulation of Tbx21ChR2-tdTomato (ChR2) and Tbx21-Cre-negative (control) animals depicted as time spent per area (border versus centre; two-way ANOVA, Sidak post hoc; n = 3 control mice, n = 4 ChR2 mice) and total distance travelled (unpaired two-tailed Student’s t-test; n = 3 control mice, n = 4 ChR2 mice). Data are represented as mean ± s.e.m. **P ≤ 0.01. Mouse images in a–c adapted from SciDraw under a Creative Commons license CC BY 4.0.
