Fig. 5: Quantification of IL-21 in ex vivo PBMCs from patients with SjD. | Nature Metabolism

Fig. 5: Quantification of IL-21 in ex vivo PBMCs from patients with SjD.

From: Lactate signalling leads to aggregation of immune-inflammatory hotspots and SLC5A12 blockade promotes their resolution

Fig. 5

a, Schematic representation of the IL-21 quantification workflow from ex vivo supernatant. b, Levels of IL-21 in culture supernatant of PBMCs from patients with SjD with SG ELS (n = 15) and from healthy donors (n = 12), stimulated in the absence and presence of lactate (10 mM). Lines connect dots representing PBMC samples treated with lactate or left untreated from the same donor. Statistical analysis was performed using the Wilcoxon signed-rank test (*P < 0.05, **P < 0.01). c, Levels of IL-21 in culture supernatant of PBMCs from patients with SjD, stimulated with lactate (10 mM) and treated with either anti-SLC5A12 3C7 mAb or its isotype control. Lines connect dots representing PBMC samples treated with anti-SLC5A12 mAb and its isotype control from the same donor. Statistical analysis was performed using the Wilcoxon signed-rank test; P value (two-sided) is shown (*P < 0.05).

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