Extended Data Fig. 1: Gene expression and correlation analysis of inflammatory mediators in salivary glands. | Nature Metabolism

Extended Data Fig. 1: Gene expression and correlation analysis of inflammatory mediators in salivary glands.

From: Lactate signalling leads to aggregation of immune-inflammatory hotspots and SLC5A12 blockade promotes their resolution

Extended Data Fig. 1

(a) Expression levels of selected genes related to inflammatory mediators involved in ectopic lymphoid structure (ELS) formation and function in murine salivary glands collected at 0-, 5-, and 12-days post-cannulation. Adjusted p-values calculated by DESeq2 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). (b) Flow cytometry gating strategy showing the phenotype of CD4+ T cells producing IL21 (top panel). Frequency of IL21-producing T CD4+ cells with a Tfh-phenotype (CD4 + / CXCR5 + , bottom left panel), and frequency of IL21+ cells in the Tfh cell population (CD4 + / CXCR5 + /PD1 + , bottom right panel) in murine salivary glands collected at 0-, 5-, and 12-days post-cannulation.Data are expressed as mean ± SD from n = 13 glands per group. (c) Unsupervised heatmap of differentially expressed genes (DEGs) comparing SjD patients versus sicca controls. (d) Immunohistochemistry staining of human kidney tissue using anti-SLC5A12 antibody. (e) Correlation plots of SLC5A12 expression with inflammatory mediator genes relevant to ELS formation and function. Gene expression was evaluated using real-time PCR (n = 18). Spearman correlation coefficient (r) and p-values (two-sided) are shown.

Back to article page