Extended Data Fig. 6: Dynamic changes in NO production in relation to dynamic changes in nucleotide metabolism upon LPS + IFNγ stimulation.
a) Relative intracellular abundance of citrulline in BMDMs over a timecourse of continual LPS + IFNγ stimulation. Data are presented as log2 fold change compared to abundance in unstimulated cells (0 h). b) Transcriptional changes of Nos2 and Tyms in BMDMs over a timecourse of acute LPS + IFNγ stimulation. Relative expression is displayed as log2 fold change compared to unstimulated cells (0 h). c) Relative intracellular abundance of citrulline in wildtype or Nos2−/− BMDMs over a timecourse of continual stimulation. N.d. indicates not detected. d) Fraction of M + 1 labeled glutamine from 24 h γ-15N-glutamine labeling in unstimulated or 48 h continually stimulated wildtype or Nos2−/− BMDMs, with or without treatment of 200 µM NO donor DETA-NONOate throughout the timecourse. e) Relative intracellular AICAR abundance in BMDMs over a timecourse of continual stimulation. a–e) Mean ± SD (n = 3 independent samples). e) Statistical analysis was performed using one-way ANOVA followed by post hoc Dunnett’s test comparing all groups to unstimulated cells (0 h). ns indicates not significant (p > 0.01).
