Extended Data Fig. 9: HGPRT knockout suppresses macrophage migration.
a) Gene ontology enrichment of the pathways that are most downregulated in Hprt1 KO macrophages compared to wildtype. b) Schematic of a macrophage migration assay. c) Migration of wildtype and Hprt1 KO (2 clones) RAW 264.7 cells across a Matrigel-coated transwell towards C5a. Mean ± SD (n = 5 independent samples). d) Labeled fraction of left: 3PG (M + 3) and right: serine (M + 3) in wildtype or Hprt1 KO (2 clones) RAW 264.7 cells over a timecourse of continual LPS + IFNγ stimulation. Cells were labeled with U-13C-glucose for 24 h before analysis. e) Relative intracellular abundance of 3-phosphoserine in wildtype or Hprt1 KO (2 clones) RAW 264.7 cells over a timecourse of continual stimulation. f–g) Transcript levels of (f) Phgdh, Psat1, Psph, and (g) Shmt1, and Shmt2 in unstimulated or 48 h continually stimulated wildtype or Hprt1 KO RAW 264.7 cells. d–g) Mean ± SD (n = 3 independent samples). c, f–g) Statistical analysis was performed using unpaired two-tailed student’s t-test comparing wildtype to Hprt1 KO. ns indicates not significant (p > 0.05). d–e) Statistical analysis was performed using one-way ANOVA followed by post hoc Tukey’s test. Bars with different lower-case letters (a, b, or c) indicate a statistically significant difference with p < 0.01.
