Extended Data Fig. 10: Various fates of nucleotides in unstimulated or LPS + IFNγ stimulated macrophages, and in macrophages infected with T. gondii. | Nature Metabolism

Extended Data Fig. 10: Various fates of nucleotides in unstimulated or LPS + IFNγ stimulated macrophages, and in macrophages infected with T. gondii.

From: Classically activated macrophages undergo functionally significant nucleotide metabolism remodelling driven by nitric oxide

Extended Data Fig. 10

a) Schematic showing T. gondii replication in a macrophage and its dependence on salvaging host purine. Specifically identified is the parasitophorous vacuole that separates host cell cytosol from T. gondii. b–c) Labeled fractions of (b) guanosine and adenosine units from digested RNA, compared to (c) free GTP and ATP, after 24 h U-13C-glucose labeling in unstimulated or 24 h continually stimulated wildtype BMDMs. d–e) Labeling kinetics of intracellular (d) NAD+ (M + 4) and (e) NADP+ (M + 4) compared to their precursor ATP (M + 4) in unstimulated or 48 h stimulated BMDMs supplemented with 50 µM U-15N-inosine for 0–4 hours as indicated on x-axis. Mean ± SD (n = 3 independent samples). Statistical analysis was performed with unpaired two-tailed student’s t-test comparing unstimulated and stimulated cells at the final timepoint.

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