Extended Data Fig. 1: Reprogramming of nucleotide metabolism is independent of changes in cell proliferation or viability.
a) Relative cell number of BMDMs over a timecourse with or without continual stimulation. Mean ± SD (n = 4 independent samples). b) Cell viability in a population of BMDMs that are unstimulated and cultured for 72 h (n = 4 independent samples), continually stimulated for 72 h (n = 4), or acutely stimulated for 96 h (n = 3). Mean ± SD. c) Metabolomic changes in pentose phosphates, nucleotides, nucleosides, and nitrogenous bases in RAW 264.7 cells over a timecourse of continual stimulation. d) Relative cell number of RAW 246.7 cells over a timecourse with or without continual stimulation. Mean ± SD (n = 3 independent samples). e) The top 10 genes enriched along principal component 1 of the transcriptomic changes in BMDMs over a timecourse of acute stimulation. c, e) Relative (c) metabolite abundances or (e) gene expression are compared to unstimulated cells (0 h) and displayed on a heatmap, with saturating color representing 10-fold change (or 3.32 on log2 scale). Each box represents the mean of n = 3 independent samples. a, d) Statistical analysis was performed with unpaired two-tailed student’s t-test comparing unstimulated to stimulated conditions at 72 h. b) Statistical analysis was performed using one-way ANOVA followed by post hoc Dunnett’s test comparing all groups to unstimulated cells. ns indicates not significant (p > 0.01).
