Fig. 1: d-Cys impairs proliferation of certain tumour cells.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
a, Colony formation assays of A549 or MDA-MB-231 cells cultured for 15 days in medium supplemented with 100 µM d-Cys, 100 µM l-Cys or water (w/o). b, Time course of A549 cell proliferation in the absence (w/o) or presence of 500 µM d-Cys for 3 days (n = 58 biological replicates), analysed by Wilcoxon matched-pairs signed-rank test. c, Relative cell yield of BEAS-2B cells after 3 days in culture in the absence (w/o) or presence of 500 µM d-Cys; n = 8, paired two-tailed Student’s t-test. d,e, Cell cycle analysis of A549 cells cultured in the presence of indicated concentrations of d-Cys, using BrdU immunofluorescence assay (d; w/o and 500 µM d-Cys, n = 3 biological replicates each; 50, 100 and 500 µM d-Cys, n = 2 biological replicates each; two-way analysis of variance (ANOVA) followed by Dunnett’s multiple-comparisons test) or the FUCCI assay (e; n = 2 biological replicates). All data are presented as the mean ± s.d. Representative images are shown.
