Extended Data Fig. 4: Proteomic analysis of A549 cells cultured in the presence or absence of D-Cys or L-Cys.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
A549 cells were cultured for three days in DMEM containing 200 μM L-cystine (w/o) or in the same medium supplemented with either 500 μM D-Cys or L-Cys, and a proteomic analysis was performed. a, Volcano plot showing proteins that vary among the different conditions. Different comparisons have been made according to the protein colour code shown in the box. b, List of the most downregulated mitochondrial proteins in D-Cys treated cells (orange dots from (a)). See also Suppl. Table S2 for a more detailed list of proteins analysed and their comparison among the different culture conditions. c, Immunoblot analyses of representative OXPHOS protein subunit levels in indicated cell lines cultured in the absence (w/o) or presence of D-Cys. VDAC1 served as loading control. Representative blots of n = 4 (for A549 cells) and n = 2 (for MDA-MA-231 and BEAS-2B cell) biological replicates are shown.
