Fig. 2: d-Cys is imported through xCT/CD98.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
a, Outline of the pooled guide RNA (gRNA) depletion screen. b,c, Volcano plot representation of the screen hits after cell growth in the presence or absence of 500 mM d-Cys. q values were calculated using MAGeCK, which includes a correction for multiple comparisons. The most notable hits required for d-Cys toxicity are shown in c. See also Supplementary Table 1 for detailed results. FC, fold change; FDR, false discovery rate. d, Colony formation assays of control (Ctrl) A549 cells and indicated A549 KO cells in the absence (w/o) or presence of 100 μM d-Cys. e, Relative cell yield of control A549 cells and indicated A549 knockout (KO) 2D cell cultures grown for 72 h in the absence (w/o, set to 100%) or presence of 500 μM d-Cys, n = 2 biological replicates (consisting of three technical replicates each). f, Effects of erastin (Era) and d-Cys on A549 cell yield after 72 h of culture (n = 4 biological replicates), analysed by two-way ANOVA followed by Tukey’s multiple-comparisons test. g, BEAS-2B cells inducibly overexpressing xCT-Flag alone or together with CD98-Flag (Extended Data Fig. 3c) following doxycycline (Dox) addition were cultured in the absence (w/o) or presence of 500 μM d-Cys for 72 h and counted (n = 3 biological replicates), analysed by two-way ANOVA followed by Tukey’s multiple-comparisons test. Data in e–g are presented as the mean ± s.d.
