Fig. 6: D-Cys impairs mammary tumour growth in the mouse.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
a–c, MDA-MB-231 cells were implanted orthotopically into the mammary gland of immunodeficient mice. When tumours reached between 50 mm3 and 100 mm3, mice were randomized and either did not receive d-Cys (Veh, n = 5 animals; a) or were administered d-Cys (n = 6 animals; b) as explained in Methods, and the tumour growth was monitored three times per week. The experiment was stopped at day 37 after tumour engraftment, when tumours had reached 1,000 mm3 in a majority of mice. The average tumour growth (mean ± s.d.) in vehicle-treated or d-Cys-treated mice at each timepoint is summarized in c. Data were analysed by two-way ANOVA followed by Sidak’s multiple-comparisons test. d, Average tumour volume (mean ± s.d.) of the mice from (a–c) at day 33 after tumour engraftment. Data were analysed by an unpaired two-tailed Student’s t-test. e, Survival curve of vehicle-treated or d-Cys-treated mice from a–c until the end of the experiment (day 37). Data were analysed by log-rank (Mantel–Cox) test.
