Extended Data Fig. 1: D-Cys impairs proliferation of a number of different cancer cell types grown under 2D or 3D conditions.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
a, Colony formation assay of A549 cells in the absence (w/o) or presence of either the L- or D-enantiomer of each proteinogenic amino acid. Each L- or D-amino acid was added to standard medium at similar concentrations as those already present in the medium. D-Cys was used at 100 µM. b, Colony formation assay for various cell types, including BZR, LuCa62, Calu1, JL1, ZL34, and A375 cells cultured in the absence (w/o) or presence of 100 µM D-Cys. c, d, Growth of A549 (c) and LuCa62 (d) spheroids in the absence (w/o) or presence of indicated D-Cys concentrations. All data are presented as mean ± SD of n = 3 biological replicates. Spheroid volumes at the indicated days of treatment were compared by 2-way ANOVA and Dunnett’s multiple comparisons test; e, Colony formation assays for HCT-116, DLD-1, HeLa and H2052/484 tumour cells in the absence (w/o) or presence of D-Cys. f, Determination of IC50 values using nonlinear fit, inhibitor conc vs response, for D-cystine or D-Cys for growth of A549 (left) or MDA-MB-231 cells (right).
