Extended Data Fig. 3: xCT (SLC7A11) and CD98 (SLC3A2) expression is associated with D-Cys sensitivity.
From: d-cysteine impairs tumour growth by inhibiting cysteine desulfurase NFS1
a, b, Immunoblotting of xCT and CD98 in various cell types that display sensitivity (a) or resistance (b) to D-Cys. c, Doxycycline-induced (co-)expression of C-terminally FLAG-tagged xCT and CD98 was analysed by anti-FLAG immunoblotting in BEAS-2B cells stably transduced by lentiviral infection. d, HeLa cells were transiently transfected to co-express C-terminally FLAG-tagged xCT and CD98, or the reference protein EGFP as indicated, cultured in absence (w/o) or presence of D-Cys for three days, and analysed by anti-FLAG immunoblotting. e, Protein yield of HeLa cell cultures from (d). Data are presented as mean ± SD; *P < 0.05; ns, not significant. Comparison by 2-way repeated measures ANOVA and Bonferroni posttest; symbols indicate matching samples of n = 4 biological replicates (one series lacks xCT/CD98 values). Representative blots of n = 4 (a, b), n = 1 for (c) and n = 4 (d) biological replicates are shown. γ-actin and β-tubulin (TUBA) were used as loading controls (molecular masses in parentheses).
