Extended Data Fig. 8: Free cholesterol overload induces microglial activation and myelin injury.
a, b, Free cholesterol (FC) injection induced increased microglial activation (a) and myelin damage (b) during the chronic stage post-injection, compared to DMSO-treated controls. Scale bar, 100 µm; n = 3 mice for the DMSO group; n = 4 mice for the FC group. c, Changes in cholesterol metabolism-associated gene expression following cholesterol crystal (CC) injection, demonstrating molecular alterations in response to CC overload. n = 3 mice/group. Data are presented as mean ± SD. Statistical significance was assessed by a two-sided unpaired Student’s t-test (a, b).
