Extended Data Fig. 9: Reproductive strategies and verification of Cyp46a1 transgenic mice.
a, Breeding strategy for generating Cyp46a1 overexpression of transgenic mice, illustrating the genetic approach used for microglia-specific overexpression. b, Double immunostaining for IBA1 and CYP46A1 confirms elevated Cyp46a1 expression in microglia of Cyp46a1-cKI mice compared to controls. Scale bar, 20 µm. Cyp46a1 cKI, n = 42 cells from three mice; control, n = 35 cells from three mice. c-e, Immunofluorescence analysis demonstrating microglia-specific expression of Cyp46a1 in cKI mice, as shown by co-localization of IBA1⁺ (microglia marker) and tdTomato⁺ signals. Main image: scale bar, 500 µm; higher-magnification panel: scale bar, 50 µm (c). Scale bar, 20 µm(d); n = 3 mice/group. f, Bulk RNA-seq of CD11b⁺ cells sorted from control and Cyp46a1-cKI mice after MCAO reveals transcriptomic alterations associated with Cyp46a1 overexpression. Cyp46a1-cKI, n = 3 mice; control, n = 4 mice. Data are presented as mean ± SD. Statistical significance was assessed by a two-sided unpaired Student’s t-test (b, e, f).
