Extended Data Fig. 2: Overview of selected zonation trajectories across the human cohort.

Representative microscopy images of liver sections from 18 individuals (healthy (N = 14) and diseased (N = 4)) highlighting hepatocytes along the chosen portal-central vein trajectories, which were subsequently isolated and measured by LC-MS (colored contours overlaid on phalloidin images). The color gradient indicates the spatial ratio S from a central vein (purple, S = 0) to the corresponding portal vein (yellow, S = 1). PV: portal vein, CV: central vein. Scale bars, 50 μm.