Extended Data Fig. 5: IFN genes are upregulated by DNMT1 and IDH inhibitors.
From: Evolving cell states and oncogenic drivers during the progression of IDH-mutant gliomas
a. Box plots depict module scores (Seurat) for gene correlates of DNA methylation loss in IDH-mutant glioma organoids treated with DNMT1 inhibitor or control (n=3 technical replicates). Boxes depict 25th, 50th and 75th percentiles, and whiskers depict extreme values. b. Running Enrichment Score (GSEA) for IFNa genes regulated by DNMT1 inhibitor. c. Box plots depict module scores (Seurat) for gene correlates of methylation loss in IDH-mutant gliomas resected from patients treated with IDH inhibitors. Left: paired pre- and post-treatment samples from a single patient. Right: unpaired samples from 6 untreated and 2 treated patients. Two-tailed t-test P values: ***p<0.001. d. Running Enrichment Score (GSEA) for IFNa genes in IDH-mutant gliomas resected from patients treated with IDH inhibitor (as in c). e. Multivariate survival analysis for high-grade IDH-mutant gliomas (grade 3 and 4) stratified by NPC enrichment score and tumor grade. Hazard ratios (HR) and P-value associated with NPC-enriched tumors are indicated.
