Extended Data Fig. 3: CD47-LLO promotes dendritic cell phagocytosis, lysosomal permeabilization, antigen presentation, and cGAS-STING activation in vitro.
a, Flow cytometry analysis and b, quantification of phagocytic activity of bone marrow-derived dendritic cells (BMDCs) as evaluated by flow cytometry. BMDCs were collected from n = 3 C57BL6 mice. c, Flow cytometry analysis and d, quantification of BMDCs stained with acridine orange. BMDCs were collected from n = 4 C57BL6 mice for IgG treatment and n = 3 for remaining treatment groups. e, Flow cytometry analysis and f, quantification of cross-presentation of SIINFEKL–H2Kb peptides on the surfaces of BMDCs (n = 3). g, Flow cytometry analysis and h, quantification of pSTING levels in BMDCs isolated from co-cultures with EO771 cells. n = 4 C57BL6 mice. Data shown represent mean ± s.d. (b, d, f, h) analyzed by one-way analysis of variance with Tukey’s multiple comparisons test.
