Extended Data Fig. 7: Tumour-associated neutrophils are enriched in CD47-LLO tumours.
a, Representative images show levels of Ly-6g+ (top) and Ly-6g + /Cd11b+ (bottom) cells detected by immunostaining in 4T1Br4 breast tumour frozen tissue sections. Scale bar, 10 μm. b, Quantification of Ly-6g+ cells per DAPI+ cells per field of view (n = 15 per treatment condition from n = 3 biologically independent tumours per condition). c, Uniform manifold approximation and projection (UMAP) of only CD45+ granulocytes color-coded by sample from IgG (n = 3), anti-CD47 (n = 4), or CD47-LLO (n = 4) treated tumours. d, UMAP projection of only CD45+ granulocytes color-coded by cluster. e, Numbers of cells (y-axis) from each cluster (x-axis) color-coded by sample. f, Percentage of cells (y-axis) from each cluster (x-axis) color-coded by sample. g, Dot plot depicting the top 5 differentially expressed genes per granulocyte cluster. The dot size indicates the percentage of cells in each cluster expressing the gene and colors indicate the average expression levels. Data shown represent mean ± s.d. (b) analyzed by two-sided unpaired Student’s t test.
