Extended Data Fig. 9: Tumour-infiltrating CD8 T cells show decreased PD-1 positivity with CD47-LLO and anti-PD-1 treatment.
a, Schema for generating in vivo syngeneic orthotopic breast cancer models for intraperitoneal injection of CD47-LLO, anti-CD47, and anti-PD1. b, Flow cytometry analysis and c, quantification of PD-1 negativity in CD8 T cells isolated from 4T1Br4 tumours treated with anti-CD47, CD47-LLO, anti-CD47 + anti-PD-1, or CD47-LLO + anti-PD-1. Data show mean ± s.d. (c) analyzed by one-way analysis of variance with Tukey’s multiple comparisons test. Data show n = 4 mice per treatment group.
