Extended Data Fig. 1: Conjugation of anti-CD47 and IgG1 to Listeriolysin O.
a, Results of sheep red blood cell (sRBC) haemolysis assay of purified LLO. Data show n = 3 biologically independent experiments. b, The MIAP410 antibody (BioXCell, Cat#BE0283) or the IgG1 antibody (BioXCell, Cat#BE0083) was dialyzed against phosphate-buffered saline (PBS) buffer, pH 7.2, and the dialyzed antibody was then modified with the click chemistry labelling reagent DBCO-PEG4-NHS ester (Conju-Probe, SKU# CP-2028) for 30 min at room temperature. The crosslinker in excess was removed by dialysis against PBS buffer, pH 7.2. c, The buffer of Listeriolysin O protein (Genscript) was exchanged with PBS buffer, pH 7.2, by PD-10 column (GE healthcare, Cat# 17085101), followed by modification with the click chemistry crosslinking reagent SPDP-PEG11-azide (BroadPharm, Cat# BP-25143). d, SDS-PAGE results of IgG-LLO conjugate in reductive and non-reductive loading buffers. Representative gel of n = 4 biological replicates shown.
