Extended Data Fig. 1: Intracellular c-di-GMP concentrations across Salmonella strains.
From: Engineered bacteria for near-infrared light-inducible expression of cancer therapeutics
a, Schematic depiction of the experimental procedure and schedule for evaluating the intracellular c-di-GMP concentrations throughout different Salmonella strains. b, Intracellular c-di-GMP concentrations in WT (S. enteritidis 3934), ∆XII (S. enteritidis 3934 ∆XII), and ∆XIV (S. enteritidis 3934 ∆XIV). c, NETMAP-engineered ∆XIV (∆XIV-NETMAP) cells were exposed to NIR light (710 nm, 1 mW cm-2) for two hours. After growth for nine hours, cells from different groups were harvested and ultrasonically crushed for ELISA assay. Data (b, c) are presented as mean ± s.d.; n = 3 independent experiments. Statistical analyses in b were performed using one-way ANOVA followed by Tukey’s test with multiple comparisons. Statistical analyses in c were performed using a two-tailed unpaired t-test.
