Extended Data Fig. 2: KG2032 reacts with a subset of polymorphic HLA-DRB1 molecules, specifically those in which the 86th amino acid was not aspartic acid.

a. Flow cytometric analysis of the reactivity of KG2032 and L243 (a known anti-HLA-DR mAb) toward K562 cells expressing different HLA-DRB1 molecules together with HLA-DRA. KG2032-reactive HLA-DRB1 alleles are shown in red and KG2032-nonreactive alleles are shown in blue. Data are representative of two independent experiments. b. Comparison of the amino acid sequences of HLA-DRB1*04:05 and DRB1*15:02. c. Amino acid sequences of region 2 in the KG2032-reactive or nonreactive HLA-DRB1 alleles.