Extended Data Fig. 3: KG2032 reactivity in various subsets of normal hematopoietic cells.
a. Flow cytometric analysis of the reactivity of KG2032 or L243 (a known anti-HLA-DR mAb) to subsets of PBMCs from healthy donors. KG2032-reactive HLA-DRB1 alleles are shown in red and KG2032-nonreactive alleles are shown in blue. A total of 10 donors were analyzed. Six donors carried KG2032-reactive HLA-DRB1 alleles and 4 donors did not. The results of two representative donors in each group are shown (donors 3 and 4: KG2032-reactive, donors 5 and 6: KG2032-nonreactive). The analysis of another donor in each group is shown in Fig. 3a. b. Flow cytometric analyses of the reactivity of KG2032 or L243 to each subpopulation of normal BM cells. HSC: hematopoietic stem cells, HPC: hematopoietic progenitor cells. A total of 3 donors were analyzed and the result of a representative donor is shown. The analysis of another donor is shown in Fig. 3d. In a and b, a single analysis was performed for each sample. c. Frequencies of KG2032- or L243-positive cells in each subpopulation of normal BM cells (n = 3). d. Flow cytometric analyses of the reactivity of KG2032 or L243 to each subpopulation of normal PBMCs. cDC: conventional dendritic cells, pDC: plasmacytic dendritic cells. e. Frequencies of KG2032- or L243-positive cells in each subpopulation of normal PBMCs (n = 3). f. Flow cytometric analyses of the reactivity of KG2032 or L243 to CD34+ CB cells stimulated with the indicated concentrations of IFN-γ for 24 h. g. Frequencies of KG2032- or L243-positive cells in CD34+ CB cells stimulated with the indicated concentrations of IFN-γ (n = 3). h. Flow cytometric analyses of the reactivity of KG2032 or L243 to subpopulations of PBMCs stimulated with the indicated concentrations of IFN-γ for 24 h. i. Frequencies of KG2032- or L243-positive cells in each subpopulation after stimulation with the indicated concentrations of IFN-γ (n = 3). In b–i, three donors carrying various KG2032-reactive HLA-DRB1 alleles were analyzed, and representative flow cytometric analyses are shown. In c, e, g and i, data are presented as means ± s.e.m.
