Extended Data Fig. 4: KG2032 binds minimally to nonhematopoietic tissues carrying KG2032-reactive HLA-DRB1 alleles, even under severe inflammation.
a. Flow cytometric analysis of KG2032 or L243 reactivity to intestinal epithelial cells or lamina propria B cells obtained from the nontumorous regions of surgically resected small bowels of patients with colon cancer. SB, small bowel, pt., patient. b. Flow cytometric analysis of KG2032 or L243 reactivity to microglial cells obtained from surgically resected brain tissues of patients with nonmalignant brain diseases. (Pt. 2: focal cortical dysplasia, Pt. 3: hippocampal sclerosis) c. Immunohistochemistry of KG2032 or L243 on frozen sections of normal human lung or endometrial tissues. Representative data from one of three patient samples for each tissue are shown. Scale bars, 20 mm. d. KG2032 or L243 reactivity to lung epithelial cell line H1975 treated with IFN-γ for 48 h. Data are representative of two independent experiments. e. Flow cytometric analysis of KG2032 or L243 reactivity to intestinal epithelial cells obtained from surgically resected small bowels of patients with inflammatory bowel disease (IBD: Crohn’s disease). In a, b and e, data are representative of three donors or patients. Analysis of another donor and patient is shown in Fig. 3e, f, h. f, g. Flow cytometric analysis of KG2032 or L243 reactivity to Lin−CD127+CD161+ cells, which are enriched with innate lymphoid cells, in an unaffected region of surgically resected colon (f) before and (g) after IFN-γ stimulation for 24 h. Data are representative of two samples. KG2032-reactive HLA-DRB1 alleles are shown in red and KG2032-nonreactive alleles are shown in blue.
